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Flagellin-PAc Fusion Protein Inhibits Progression of Established Caries. 

Abstract: Dental caries remains one of the most common infectious diseases of humankind, which develops slowly throughout life, affecting children, adolescents, and adults. A vaccine against caries is urgently needed. We previously developed recombinant flagellin as a mucosal adjuvant for anti-Streptococcus mutans vaccines by nasal immunization. Furthermore, we demonstrated a fusion protein strategy that combined flagellin and the target surface adhesion protein (PAc) in a single construct. This construct enhanced specific IgA responses in oral fluids and provided improved prophylactic protection against caries. In the present study, we observed prolonged progression of dental caries in rats after S. mutans Ingbritt challenge. In addition, we observed a therapeutic effect of the flagellin-PAc fusion protein (KFrPAc) against dental caries as a mucosal vaccine with a new immunization protocol. The present study demonstrated that KF-rPAc by nasal immunization can promote PAc-specific systemic and mucosal antibody responses and inhibit dental caries progression efficiently after the implant of S. mutans into the oral cavity of the rats. The rats immunized with KF-rPAc exhibited 53.9% caries reduction compared with the sham-immunized rats. Our data support the concept of administration of KF-rPAc to humans after infection and even caries that has begun to alleviate caries progression. In conclusion, our study demonstrated that KF-rPAc could be used as an anticaries therapeutic mucosal vaccine.

sjimago:Q1 

 factor de impacto: 4.688 

 número de publicaciones del autor: 36 

 citaciones del artículo: 0 

 Año: 2015 

 revista: Journal dental research 

Construction and Immunogenic Characterization of a Fusion Anti-caries DNA Vaccine against PAc and Glucosyltransferase I of Streptococcus mutans 

Abstract: Glucosyltransferases (GTFs) and A cell-surface protein (PAc) are two important virulence factors of the cariogenic organism Streptococcus mutans. They may mediate sucrose-independent or sucrose-dependent attachment of Streptococcus mutans to tooth surfaces, respectively. Thus, inhibiting both virulence factors is predicted to provide better protection against caries than inhibiting a single factor. To develop a highly efficient vaccineagainst caries, we constructed a fusion DNA vaccine, pGLUA-P, by cloning the GLU region of GTF into a DNA vaccine, pCIA-P, which encodes two highly conservative regions of PAc. In this report, we provide evidence that fewer caries lesions were observed in rats following subcutaneous injection of pGLUA-P, compared with pCIA-P, near the submandibular gland. Our findings suggest that a multigenic DNA vaccine may be morecaries-preventive than a single-gene DNA vaccine.

 scjimago: Q1 

 Factor de impacto : 4.139 

 Numero de publicaciones del autor: 30 

 Citaciones del articulo:46 

 Revista: Journal dental research 

The control of dental caries: can vaccines prevent cavities? 

Abstract: Essentially, responsibility for developing and implementing measures designed to control dental caries should rest with the dental profession. Many of the most effective ways of controlling caries, however, involve the use of fluoride. Either systemically via fluoridated water and tablets or, topically, with fluoride-containing toothpaste and mouthrinses. Scientists in many disciplines are aware that fluorides can, in certain circumstances, endanger the environment. There is some evidence that fluoride contamination of the total environment has increased in recent years. The usefulness of fluoride as a caries preventive does not mean that unnecessary exposure to the element should be tolerated. It is conceivable that fluoride is approaching the limits of its effectiveness as a caries preventive. With this possibility in mind, recent research directed toward the development of safe, effective anti-tooth decay vaccines has been reviewed.

 Revista: The Science of the total environment 

 Factor de impacto:4.099 

 Scimago:Q1. 

 Autor: Smith GE 

 publicaciones del autor:478. 

 Citaciones del artículo: 2  

The scientific and public-health imperative for a vaccine against dental caries 

Abstract: Dental caries is caused by one of the most ubiquitous bacterial infections of humans. In many countries such as Brazil and China, this disease is reaching epidemic proportions, and it is clear that a more effective public-health measure to combat dental caries is needed, because disadvantaged children are the most severely affected. One of the main groups of oral microorganisms, the mutans streptococci, has been associated with the aetiology of dental caries, and preclinical studies of immunological interventions have shown the feasibility of interfering with this disease. Moreover, clinical trials have indicated that a mucosal immune response to a crucial antigen(s) of mutans streptococci can influence the pathogenesis of dental caries. Evidence that this antigen(s) is appropriate for use in a vaccine against dental caries, as well as evidence for an appropriate target population of individuals and a logical time of administration, has now emerged.

 factor de impacto: 

 índice de citaciones del artículo: 20 

 numero de publicaciones: 81 

 quartil: Q1

 autor : Taubman, Martin A; Nash, David A 

 año: 2006 

Construction of a new fusion anti-caries DNA vaccine. Construcción de una vacuna de ADN anti- caries nueva fusión 

Abstract: Mutans streptococci (MS) are generally considered to be the principal etiological agent of dental caries. MS have two important virulence factors: cell- surface protein PAc and glucosyltransferases (GTFs). GTFs have two functional domains: an Nterminal catalytic sucrose-binding domain (CAT) and a C-terminal glucan-binding domain (GLU). A fusion anti-caries DNA vaccine, pGJA-P/VAX, encoding two important antigenic domains, PAc and GLU, of S. mutans, was successful in reducing the levels of dental caries caused by S. mutans in gnotobiotic animals. However, its protective effect against S. sobrinus infection proved to be weak. Does the DNA vaccine need an antigen of S. sobrinus to enhance its ability to inhibit infection? To answer this question, in this study, we cloned the catalytic (cat) fragment of S. sobrinus gtf-I, which demonstrated its ability to inhibit water-insoluble glucan synthesis by S. sobrinus, into pGJA-P/VAX to produce a new anti-caries DNA vaccine. 

 factor de impacto: 4.688 

 índice de citaciones del artículo: 19 

 número de publicaciones: 41 

 quartil: Q1 

 autor :Niu, Yongsheng

Current status of a mucosal vaccine against dental caries 

Abstract: The evidence of a specific bacterial cause of dental caries and of the function of the salivary glands as an effector site of the mucosal immune system has provided a scientific basis for the development of a vaccine against this highly prevalent and costly oral disease. Research efforts towards developing an effective and safe caries vaccine have been facilitated by progress in molecular biology, with the cloning and functional characterization of virulence factors from mutans streptococci, the principal causative agent of dental caries, and advancements in mucosal immunology, including the development of sophisticated antigen delivery systems and adjuvants that stimulate the induction of salivary immunoglobulin A antibody responses. Cell-surface fibrillar proteins, which mediate adherence to the salivary pellicle, and glucosyltransferase enzymes, which synthesize adhesive glucans and allow microbial accumulation, are virulence components of mutans streptococci, and primary candidates for a human caries vaccine. Infants, representing the primary target population for a caries vaccine, become mucosally immunocompetent and secrete salivary immunoglobulin A antibodies during the first weeks after birth, whereas mutans streptococci colonize the tooth surfaces at a discrete time period that extends around 26 months of life. Therefore, immunization when infants are about one year old may establish effective immunity against an ensuing colonization attempts by mutans streptococci. The present review critically evaluates recent progress in this field of dental research and attempts to stress the protective potential as well as limitations of caries immunization.

 Revista: molecular oral microbiology 

 Factor de impacto: 2.784 

 Scimago: Q1 

 Autor: G. Hajishengallis and S. M. Michalek 

 publicaciones del autor:135 

 Citaciones del artículo: 110  Año:1999